HYBRIDIZATION IMAGE

NORMAL

Aneusomic interphase cell obtained from a sample showing two copies of chromosome 3 (red), four copies of chromosome 7 (green), five copies of chromosome 17 (aqua) and one copy of p16 gene (gold) after the UroVysion Bladder Cancer Kit (UroVysion Kit) hybridization.

ABNORMAL

Nucleus showing the one aqua/orange, one green, and one orange/green fusion (yellow) signal pattern.

NORMAL

Nucleus showing the two aqua/orange and two green signal pattern.

ABNORMAL

Nucleus showing the one aqua/orange, one green, and one orange/green fusion (yellow) signal pattern.

NORMAL

Nucleus showing the two aqua/orange and two green signal pattern.

NORMAL

Result of the hybridization of the LSI 9q34 Probe as observed in a normal interphase cell.

ABNORMAL

Abnormal cell hybridized with the LSI 9q34 Probe. The cell in this image shows deletion of one copy of the 9q34 region of chromosome 9 as indicated by the single aqua signal.

ABNORMAL

LSI BCR/ABL ES Dual Color Translocation Probe hybridized to a nucleus containing the t(9;22) showing one green (native BCR), one large orange(native ABL), one smaller orange (ES) and one fused orange/green(20IGIF) signal pattern. A nucleus lacking the t(9;22) will exhibit a two orange, two green(2O2G) signal pattern. In a nucleus possessing the t(9;22) involving the M-bcr, one green (native BCR), one large orange (native ABL), one smaller orange (ES), and one fused orange/green signal (5' BCR/3' ABL),(2O1G1F) will be observed. Minor breakpoint (m-bcr) signal patterns may appear as one orange, one green, and two fusion signals. In some cells a deletion may occur 5' of the ABL breakpoint that may reduce the ES pattern to a single fusion pattern.

ABNORMAL

LSI BCR/ABL ES Dual Color Translocation Probe hybridized to a nucleus containing the t(9;22) showing one green (native BCR), one large orange(native ABL), one smaller orange (ES) and one fused orange/green(20IGIF) signal pattern. A nucleus lacking the t(9;22) will exhibit a two orange, two green(2O2G) signal pattern. In a nucleus possessing the t(9;22) involving the M-bcr, one green (native BCR), one large orange (native ABL), one smaller orange (ES), and one fused orange/green signal (5' BCR/3' ABL),(2O1G1F) will be observed. Minor breakpoint (m-bcr) signal patterns may appear as one orange, one green, and two fusion signals. In some cells a deletion may occur 5' of the ABL breakpoint that may reduce the ES pattern to a single fusion pattern.

NORMAL

LSI BCR/ABL Dual Color, Single Fusion Translocation Probe hybridized to a nucleus containing the t(9;22). One orange, one green and one fusion (IOIGIF) signal pattern is observed.

ABNORMAL

LSI BCR/ABL Dual Color, Dual Fusion Translocation Probe hybridized to a nucleus containing a simple balanced t(9;22). One orange, one green and two orange/green fusion signals are observed (1O1G2F).A nucleus lacking the t(9;22) translocation will exhibit the two orange, two green (2O2G) signal pattern. In a nucleus containing a simple balanced t(9;22), one orange and one green signal from the normal 9 and 22 chromosomes and two orange/green (yellow) fusion signals, one each from the derivative 9 and 22 chromosomes, will be observed (1O1G2F). In some instances, deletions may occur 3' of the BCR breakpoint and/or 5' of the ABL breakpoint resulting in either an ES (extra orange or green) signal pattern or a single fusion pattern.

ABNORMAL

LSI BCR/ABL Dual Color, Single Fusion Translocation Probe hybridized to a nucleus. One orange, one green and one fusion (IOIGIF) signal pattern is observed.

NORMAL

LSI BCR/ABL Dual Color, Single Fusion Translocation Probe hybridized to a nucleus containing the t(9;22). One orange, one green and one fusion (IOIGIF) signal pattern is observed.