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Vysis FISH Chromosome Search - Chromosome 14 | Abbott Molecular

VYSIS FISH CHROMOSOME SEARCH

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TelVysion 14q SpectrumOrange

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CE Vysis IGH/CCND1 XT DF FISH Probe Kit

RESULTS OF HYBRIDIZATION

HYBRIDIZATION IMAGE

ABNORMAL

An abnormal interphase cell hybridized with the Vysis LSI IGH/CCND1 XT Dual Color Dual Fusion Probes. The cell in this image shows the one orange (CCND1/MYEOV), one green (IGH), two fusion (der (11) and der (14)) signal pattern indicative of a t(11;14). In an abnormal cell containing the t(11;14), one orange (CCND1/MYEOV), one green (IGH), and two fusion signal pattern (der (11) and der (14)) may be observed. Some samples containing the t(11;14) may display signal patterns different than one orange, one green, and two fusions.

NORMAL

A normal interphase cell hybridized with the Vysis LSI IGH/CCND1 XT Dual Color Dual Fusion Probes. The cell shows the expected two orange (CCND1/MYEOV), two green (IGH) signal pattern. In a normal cell that lacks the t(11;14), a two orange and two green signal pattern will be observed reflecting the two intact copies of CCND1/MYEOV and IGH respectively.

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CE Vysis IGH/FGFR3 DF FISH Probe Kit

RESULTS OF HYBRIDIZATION

HYBRIDIZATION IMAGE

NORMAL

An interphase cell hybridized with the LSI IGH/FGFR3 Dual Color Dual Fusion Probe. The cell shows the two orange (FGFR3),two green (IGH) signal pattern.In a normal cell that lacks the t(4;14), a two orange and two green signal pattern will be observed reflecting the two intact copies of the FGFR3 and IGH regions respectively.

ABNORMAL

An interphase cell hybridized with the LSI IGH/FGFR3 Dual Color, Dual Fusion Probe. The cell in this image shows the one orange(FGFR3), one green (IGH), two fusion (der (4) and der (14)) signal pattern.In an abnormal cell containing the t(4;14), one orange (FGFR3), one green (IGH), and two fusion signalpattern (der (4) and der (14)) may be observed. Some samples containing the t(4;14) may display signal patterns differently than one orange, one green, and two fusions.

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CE Vysis LSI IGH Dual Color, Break Apart Rearrangement Probe Kit

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ASR Vysis LSI IGH Dual Color, Break Apart Rearrangement Probe

RESULTS OF HYBRIDIZATION

HYBRIDIZATION IMAGE

NORMAL

LSI IGH Dual Color, Break Apart Rearrangement Probe hybridized to nuclei exhibiting the expected two fusion (2F) signal pattern.When hybridized to a normal nucleus, the LSI IGH Dual Color, Break Apart Rearrangement Probe produces a two orange/green (yellow) fusion (2F) signal pattern. As there is no probe targeted to the J or constant regions, a slight gap between the two differently colored probe signals may sometimes be observed in nuclei from normal cells. When the IGH Dual Color, Break Apart Translocation Probe is hybridized to a nucleus containing an IGH translocation, one orange, one green, and one orange/green fusion signal pattern is observed (1O1G1F). This signal pattern indicates that the genomic targets for the LSI IGHV and LSI IGH 3' flanking probes have been physically separated as a result of the translocation. As V(D)J rearrangements may occur on either, or both, of the translocated and non-translocated IGH alleles, the green LSI IGHV probe signal intensity on either, or both, of the alleles may be diminished as a result of probe target deletion in some samples.

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ASR Vysis LSI IGH/BCL2 Dual Color Dual Fusion Probes

RESULTS OF HYBRIDIZATION

HYBRIDIZATION IMAGE

ABNORMAL

LSI IGH/BCL2 Dual Color, Dual Fusion Translocation Probe hybridized to a nucleus containing the t(14;18) (q32;q21) showing the 1O1G2F signal pattern.The expected pattern in a normal nucleus hybridized with the LSI IGH/BCL2 probe is the two orange, two green signal pattern (2O2G). In a nucleus harboring a t(14;18), the most common pattern is one orange signal, one green signal (representing the normal homolog) and two orange/green (yellow) fusion signals representing the two derivative chromosomes resulting from the reciprocal translocation (1O1G2F pattern). Patterns other than 1O1G2F may be observed in some abnormal cells including instances of nuclei containing more than two fusion signals.

ABNORMAL

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CE Vysis LSI IGH/BCL2 Dual Color, Dual Fusion Translocation Probe Set

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CE Vysis LSI IGH/CCND1 DF FISH Probe Kit

RESULTS OF HYBRIDIZATION

HYBRIDIZATION IMAGE

ABNORMAL

LSI IGH/CCND1 Dual Color, Dual Fusion Translocation Probe hybridized to an abnormal nucleus showing the common 1O1G2F signal pattern.LSI IGH/CCND1 hybridized to a cell containing t(11;14) with breakpoints at the MTC on 11q13 and at the IGH J region on 14q32 is expected to result in a signal pattern of two orange/green (yellow) fusions, one on each of the abnormal chromosomes 11 and 14 and single orange and green signals from the normal chromosomes.Due to the gap between the two probes in the IGH probe set, the normal IGH loci may sometimes appear as two slightly separated green signals. This gap may also cause a slight separation of the orange and green signals on the der(11) chromosome, in some instances. Analysis of t(11;14) samples suggests that due to variation in breakpoint location on 11q13 loss of V segments within the LSI IGH probe target, some samples containing t(11;14) might display signal patterns different than 1O1G2F.

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ASR Vysis LSI IGH/CCND1 Dual Color Dual Fusion Probes

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ASR Vysis LSI IGH/CCND1 XT Dual Color, Dual Fusion Translocation Probes

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ABNORMAL

An interphase cell hybridized with the LSI IGH/CCND1 XT Dual Color, Dual Fusion Probes. The cell in this image shows the one orange (CCND1/MYEOV), one green (IGH), two fusion (der (11) and der (14)) signal pattern.

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The interphase cell shows the two orange (CCND1/MYEOV), two green (IGH) signal pattern.

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ASR Vysis LSI IGH/FGFR3 Dual Color Dual Fusion Probes

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An interphase cell hybridized with the LSI IGH/FGFR3 Dual Color, Dual Fusion Translocation Probe. The cell shows the two orange (FGFR3), two green (IGH) signal pattern.

ABNORMAL

An interphase cell hybridized with the LSI IGH/FGFR3 Dual Color, Dual Fusion Translocation Probe. The cell in this image shows the one orange (FGFR3), one green (IGH), two fusion (der (4) and der (14)) signal pattern.

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CE Vysis LSI IGH/MAF DF FISH Probe Kit

RESULTS OF HYBRIDIZATION

HYBRIDIZATION IMAGE

ABNORMAL

"An interphase cell hybridized with the LSI IGH/MAF Dual Color, Dual Fusion Translocation Probe. The cell in this image shows the one green (IGH), one orange (MAF), two fusion (der (14) and der (16)) signal pattern indicative of a y(14;16). "

NORMAL

An interphase cell hybridized with the LSI IGH/MAF Dual Color, Dual Fusion Translocation Probe. The cell shows the two green (IGH), two orange (MAF) signal pattern. In a normal cell that lacks the t(14;16), a two green and two orange signal pattern will be observed reflecting the two intact copies of IGH and the MAF region respectively. Due to the presence of the ~2.2 Mb gap between the two SpectrumOrange labeled MAF probes, signal splitting of the orange probe may be observed in both normal and abnormal cells.

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ASR Vysis LSI IGH/MAF Dual Color Dual Fusion Probes

RESULTS OF HYBRIDIZATION

HYBRIDIZATION IMAGE

ABNORMAL

An interphase cell hybridized with the LSI IGH/MAF Dual Color Dual Fusion Probes. The cell in this image shows the one green (IGH), one orange (MAF), two fusion (der (14) and der (16)) signal pattern.

NORMAL

An interphase cell hybridized with the LSI IGH/MAF Dual Color, Dual Fusion Translocation Probe. The cell shows the two green (IGH), two orange (MAF) signal pattern.

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ASR Vysis LSI IGH/MALT1 Dual Color Dual Fusion Probes

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CE Vysis LSI IGH/MYC/CEP 8 Tri-Color Dual Fusion Probe Kit

RESULTS OF HYBRIDIZATION

HYBRIDIZATION IMAGE

Vysis-LSI-IGH_MYC_CEP-8-Tri-Color-Dual-Fusion-Probe-Kit_Hybridization

NORMAL

LSI IGH/MYC/CEP 8 Tri-Color Dual Fusion Probes hybridized to a normal nucleus showing the expected 202G2A signal pattern.

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CE Vysis LSI TRA/D Dual Color Break Apart Rearrangement FISH Probe Kit

RESULTS OF HYBRIDIZATION

HYBRIDIZATION IMAGE

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Nucleus showing the two green/orange fusion signals.

ABNORMAL

Nucleus showing the one green/orange fusion, one green and one orange signal pattern.

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ASR Vysis LSI TRA/D Dual Color Break Apart Rearrangement Probe

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CE Vysis ToTelVysion Multi-Color FISH Probe

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ASR Vysis ToTelVysion Multi-Color FISH Probe

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Patented - Abbott Diagnostics

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VYSIS FISH CHROMOSOME SEARCH

TelVysion 14q SpectrumOrange

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Accord Semi-Automatic Scanner (Manual Stage)

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CE Vysis IGH/CCND1 XT DF FISH Probe Kit

HYBRIDIZATION IMAGE

ABNORMAL

NORMAL

PROBE INFO

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Accord Semi-Automatic Scanner (Manual Stage)

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CE Vysis IGH/FGFR3 DF FISH Probe Kit

HYBRIDIZATION IMAGE

NORMAL

ABNORMAL

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Accord Semi-Automatic Scanner (Manual Stage)

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CE Vysis LSI IGH Dual Color, Break Apart Rearrangement Probe Kit

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Accord Semi-Automatic Scanner (Manual Stage)

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ASR Vysis LSI IGH Dual Color, Break Apart Rearrangement Probe

HYBRIDIZATION IMAGE

NORMAL

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Accord Semi-Automatic Scanner (Manual Stage)

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ASR Vysis LSI IGH/BCL2 Dual Color Dual Fusion Probes

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ABNORMAL

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Accord Semi-Automatic Scanner (Manual Stage)

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CE Vysis LSI IGH/BCL2 Dual Color, Dual Fusion Translocation Probe Set

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Accord Semi-Automatic Scanner (Manual Stage)

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CE Vysis LSI IGH/CCND1 DF FISH Probe Kit

HYBRIDIZATION IMAGE

ABNORMAL

PROBE INFO

ORDERING INFO

PRODUCT INFO REQUEST

Accord Semi-Automatic Scanner (Manual Stage)

ORDER#:

ASR Vysis LSI IGH/CCND1 Dual Color Dual Fusion Probes

PROBE INFO

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ASR Vysis LSI IGH/CCND1 XT Dual Color, Dual Fusion Translocation Probes

HYBRIDIZATION IMAGE

ABNORMAL

NORMAL

PROBE INFO