Home
Abbott Header Logo
Logo Image

Home > Knowledge Center > Support > Vysis FISH Knowledge Center >  Frozen Tissue Prep for FISH

Frozen Tissue Prep For Fish

FROZEN TISSUE PREP FOR FISH image
FROZEN TISSUE PREP FOR FISH image
FROZEN TISSUE PREP FOR FISH image

Standard Protocol

  • Cut 5um sections on silanized or positively charged slides
  • Dry at room temperature for 15-30 min
  • Fix in 4% formaldehyde in PBS at 0 ºC for 15 min
  • Wash with PBS and start the Paraffin Pretreatment kit protocol at the .2N HCl step and follow through to the end

Alternate Protocol

  • Cut sections as before and dry
  • Fix in Carnoy's fixative (3:1 methanol: acetic acid) for 5 min.
  • Begin the Paraffin Pretreatment kit protocol just prior to the Protease step (Wash Buffer for 5 min.)

HELPFUL RESOURCES

Vysis FISH Chromosome Search
Microscope Filter Order Form
Contact Technical Support
Logo Image

© 2024 Abbott, Abbott Park, Illinois, U.S.A.

Unless otherwise specified, all product and service names appearing in this Internet site are trademarks owned by or licensed to Abbott, its subsidiaries or affiliates. No use of any Abbott trademark, trade name, or trade dress in this site may be made without the prior written authorization of Abbott, except to identify the product or services of the company. Other trademarks are the property of their respective owners.

This Internet site that you have requested is intended for the residents of a particular country or countries and may contain information on pharmaceuticals, medical devices and other products or uses of those products that are not approved in other countries or regions. If you are a resident of a country other than those to which this site is directed, contact your local Abbott affiliate to obtain the appropriate product information for your country of residence.

YOU ARE ABOUT TO EXIT THE ABBOTT FAMILY OF WEBSITES FOR A 3RD PARTY WEBSITE

Links which take you out of Abbott worldwide websites are not under the control of Abbott, and Abbott is not responsible for the contents of any such site or any further links from such site. Abbott is providing these links to you only as a convenience, and the inclusion of any link does not imply endorsement of the linked site by Abbott. The website that you have requested also may not be optimised for your screen size.

DO YOU WISH TO CONTINUE AND EXIT THIS WEBSITE?

YOU ARE ABOUT TO ENTER AN ABBOTT COUNTRY OR REGION SPECIFIC WEBSITE.

Please be aware that the website you have requested is intended for the residents of a particular country or countries, as noted on that site. As a result, the site may contain information on pharmaceuticals, medical devices and other products or uses of those products that are not approved in other countries or regions.

DO YOU WISH TO CONTINUE AND ENTER THIS WEBSITE?