Direct Bone Marrow Smears

DIRECT BONE MARROW SMEARS image
DIRECT BONE MARROW SMEARS image
DIRECT BONE MARROW SMEARS image
  1. Prepare bone marrow according to your lab methodology.
  2. Dip slides or coverslips into a coplin jar containing 70% acetic acid for 30 seconds at room temperature.
  3. Wash twice, briefly, in distilled water.
  4. Dehydrate successively in 70%, 85%, 100% EtOH 2 minutes each.
  5. Dip in cold acetone for 2 minutes.
  6. Air dry.
  7. Proceed with bone marrow pretreatment protocol.If slides are dropped the same day, put the slide in 2XSSC for 2 minutes at 73ºC. (step 1 of bone marrow pretreatment protocol).

*Smears stored up to 30 months (perhaps even longer) can be used successfully.